Journal: Frontiers in Neuroanatomy

Article Title: Alpha retinal ganglion cells in pigmented mice retina: number and distribution

doi: 10.3389/fnana.2022.1054849

Figure Lengend Snippet: Primary and secondary antibodies used in this work.

Article Snippet: , Rabbit anti OPN4 , AB-N39 Advanced Targeting Systems , 1:1,000.

Techniques:

Journal: Frontiers in Neuroanatomy

Article Title: Alpha retinal ganglion cells in pigmented mice retina: number and distribution

doi: 10.3389/fnana.2022.1054849

Figure Lengend Snippet: ONs-αRGCs are not immunodetected against melanopsin. Photomicrographs of flat-mounted retinas (A–A ”’ ) or radial retinal sections (B–B ” ) immunodetected against the αRGC pan-marker OPN (A,B) in blue, Calbindin (A’,B’) in green and OPN4 (A”,B”) in red (merged in A ”’ ) in the same area of the retina. White circles represent cells positive for OPN + and Calbindin + but negative for OPN4 (ONs-αRGCs/M4ipRGCs) and yellow arrowheads represent cells positive for OPN4 but negative for OPN and Calbindin (M1-M3-ipRGCs). Scale bar: 100 μm.

Article Snippet: , Rabbit anti OPN4 , AB-N39 Advanced Targeting Systems , 1:1,000.

Techniques: Marker

Journal: Investigative Ophthalmology & Visual Science

Article Title: Assessment of Rod, Cone, and Intrinsically Photosensitive Retinal Ganglion Cell Contributions to the Canine Chromatic Pupillary Response

doi: 10.1167/iovs.16-19865

Figure Lengend Snippet: Gene and protein expression patterns in dogs with inherited retinal diseases compared to those in WT dogs. ( A ) Effects of inherited retinal disease in various canine models on retinal melanopsin/ Opn4 mRNA expression levels, quantified by qRT-PCR and compared with those in age-matched WT dogs. Melanopsin/ Opn4 was variable but not significantly altered in CNGB3 -ACHM. Retinas in PRCD -mutants showed a significant decrease (**) compare with those in WT retinas ( P < 0.05). Expression levels in individual RPGR - and PDE6B -mutants also appeared decreased. ( B ) Melanopsin immunostaining of representative retinal sections from WT and PDE6B -mutant dogs confirms the presence of ipRGCs. Other RGCs are shown in green (NeuN). Cell nuclei are shown in blue with DAPI staining. Calibration bar : 20 μm. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer.

Article Snippet: A custom-made rabbit anti-canine melanopsin antibody directed at a mixture of N-terminal (NH 2 -MNPPSGPGAQEPGC-amide) and C-terminal (CAKAPLRPRGQAVETPGKV-amide) peptides (21st Century Biochemicals, Marlboro, MA, USA) was generated and affinity purified.

Techniques: Expressing, Quantitative RT-PCR, Immunostaining, Mutagenesis, Staining

Journal: Frontiers in Neuroanatomy

Article Title: Central melanopsin projections in the diurnal rodent, Arvicanthis niloticus

doi: 10.3389/fnana.2015.00093

Figure Lengend Snippet: Pituitary adenylate cyclase-activating polypeptide (PACAP) is found in several subtypes of melanopsin cells in the Nile grass rat retina. An extended view across the Z-stack of melanopsin (red) and PACAP (green; overlay in yellow) in the retina is depicted in (A) , while single labels for PACAP and melanopsin are depicted in (B,C) , respectively. In (A–C) , the two single arrowheads indicate two melanopsin-expressing cells that do not express PACAP, while the double arrowhead indicates a PACAP-expressing cell that does not express melanopsin. Extended views of the melanopsin cells are shown before (D) and after (E,F) analysis of the dendritic processes. In (D–F) the various subtypes of melanopsin-expressing cells are marked 1–6. 1 = M1, 2 and 3 = M3, 4 = M2, and 5–7 = displaced M1 cells. GCL, ganglion cell layer; IPL, inner plexiform layer; and INL, inner nuclear layer. Scale bars: 45 μm.

Article Snippet: A rabbit anti-melanopsin antibody [41K7; diluted 1:5,000; ( )] directed against the C-terminal of melanopsin was used together with a N-terminal rabbit anti-melanopsin antibody (PAI-780, Fisher Scientific Inc., Barrington, IL, USA; 1:5,000) to stain melanopsin cells in the retina.

Techniques: Expressing

Journal: Frontiers in Neuroanatomy

Article Title: Central melanopsin projections in the diurnal rodent, Arvicanthis niloticus

doi: 10.3389/fnana.2015.00093

Figure Lengend Snippet: The distribution of different subtypes of intrinsically photosensitive retinal ganglion cells (ipRGCs) across the Nile grass rat retina. M1 and M3 ipRGCs (dark blue) and M2 (light blue) cells are evenly distributed across the retina, whereas displaced M1 cells (green) are located primarily in its superior region (A) . A higher power image of M1 cells is shown in (B) , which represents the boxed area in the nasal region of (A) . Melanopsin cells in the superior aspect of the retina (boxed region in A ) are seen in the ganglion cell layer (GCL; C ) and in the inner plexiform layer (IPL; D ), where a dense plexus of melanopsin-containing fibers can also be seen. Scale bars: (B–D) = 50 μm

Article Snippet: A rabbit anti-melanopsin antibody [41K7; diluted 1:5,000; ( )] directed against the C-terminal of melanopsin was used together with a N-terminal rabbit anti-melanopsin antibody (PAI-780, Fisher Scientific Inc., Barrington, IL, USA; 1:5,000) to stain melanopsin cells in the retina.

Techniques:

Journal: Frontiers in Neuroanatomy

Article Title: Central melanopsin projections in the diurnal rodent, Arvicanthis niloticus

doi: 10.3389/fnana.2015.00093

Figure Lengend Snippet: Staining for melanopsin and PACAP reveals that they are expressed in the same cells in the Nile grass rat retina. Photomicrographs depict melanopsin-immunoreactive (ir) cells (A) , PACAP-ir cells (B) and the overlay of staining for PACAP and melanopsin ( C ; melanopsin-ir in red and the pixel overlap of melanopsin-ir and PACAP-ir in white). Manual counts of cells expressing melanopsin and PACAP were done in Fiji (D) . Scale bars: (A–D) = 100 μm and insert in (D) = 30 μm.

Article Snippet: A rabbit anti-melanopsin antibody [41K7; diluted 1:5,000; ( )] directed against the C-terminal of melanopsin was used together with a N-terminal rabbit anti-melanopsin antibody (PAI-780, Fisher Scientific Inc., Barrington, IL, USA; 1:5,000) to stain melanopsin cells in the retina.

Techniques: Staining, Expressing

Journal: Frontiers in Neuroanatomy

Article Title: Central melanopsin projections in the diurnal rodent, Arvicanthis niloticus

doi: 10.3389/fnana.2015.00093

Figure Lengend Snippet: Schematic diagram of the retinal projections from PACAP/melanopsin-containing RGCs (left) and RGCs not containing PACAP/melanopsin (right) in the Nile grass rat brain. The thickness of the arrows roughly indicates the density of the innervation to the various brain regions listed. Some projections of each type that are presumed to exist in other hypothalamic regions are presented with dashed lines. VLPO, ventrolateral preoptic area; LH, lateral hypothalamus; SCN, suprachiasmatic nucleus; SPVZ, subparaventricular zone; vLGN, ventral lateral geniculate nucleus; IGL, intergeniculate leaflet; dLGN, dorsal lateral geniculate nucleus; PLi, posterior limitans; OPT, olivary pretectal nucleus; SC, superior colliculus.

Article Snippet: A rabbit anti-melanopsin antibody [41K7; diluted 1:5,000; ( )] directed against the C-terminal of melanopsin was used together with a N-terminal rabbit anti-melanopsin antibody (PAI-780, Fisher Scientific Inc., Barrington, IL, USA; 1:5,000) to stain melanopsin cells in the retina.

Techniques:

Journal: PLoS ONE

Article Title: Dendritic Morphology of Caudal Periaqueductal Gray Projecting Retinal Ganglion Cells in Mongolian Gerbil ( Meriones unguiculatus)

doi: 10.1371/journal.pone.0103306

Figure Lengend Snippet: (A–C) Illustration of the CTB-conjugated Alexa Fluor 594 (CTB-594) retrogradely labeled RGCs in the retina. There are 4 CTB-labeled RGCs (magenta) in this field (A). These cells were not visible under blue excitation florescence filter (B). Merged photomicrograph of (A) and (B) is shown in (C). (D–F) A cPAG-projecting RGC is negative for anti-melanopsin staining. Arrow points to a CTB-594 retrogradely labeled cPAG-projecting RGC (D) that, as shown in (E), lacks melanopsin immunoreactivity. In contrast, melanopsin-positive RGCs were also seen. As shown in (E), the soma and dendrites of a melanopsin-expressing RGC (mRGC) were visible, so was an dendrite of another mRGC whose soma was not in the field (green fluorescent staining). Merged photomicrograph shows a cPAG-projecting RGC and a non-cPAG-projecting but melanopsin-positive RGC in the same field (F). Scale bars: 40 µm in C (applies to A and B); 20 µm in F (applies to D and E).

Article Snippet: Then retinas were incubated for 3 days at 4°C with a rabbit anti-melanopsin antibody (PA1-780, Fisher Scientific Inc., Barrington, IL; 1∶600).

Techniques: Labeling, Staining, Expressing

Journal: PLoS ONE

Article Title: Dendritic Morphology of Caudal Periaqueductal Gray Projecting Retinal Ganglion Cells in Mongolian Gerbil ( Meriones unguiculatus)

doi: 10.1371/journal.pone.0103306

Figure Lengend Snippet: (A) Coronal section of an injection site. (B–D) Arrow points to a CTB retrogradely labeled rPAG-projecting RGC. This cell was also positive for melanopsin immunoreactivity (C), but not all mRGCs could be retrogradely labeled by CTB (arrowhead in C and D). bsc: the brachium of the superior colliculus; PiRe: pineal recess; pc: posterior commissure; rPAG: the rostral periaqueductal gray. Scale bars: 300 µm in A; 20 µm in D (applies to B and C).

Article Snippet: Then retinas were incubated for 3 days at 4°C with a rabbit anti-melanopsin antibody (PA1-780, Fisher Scientific Inc., Barrington, IL; 1∶600).

Techniques: Injection, Labeling

Journal: PLoS ONE

Article Title: Dendritic Morphology of Caudal Periaqueductal Gray Projecting Retinal Ganglion Cells in Mongolian Gerbil ( Meriones unguiculatus)

doi: 10.1371/journal.pone.0103306

Figure Lengend Snippet: (A) A photomontage of cPAG-projecting RGC. This cell lies at 4.35 mm from the optic disk. The arrowhead points to an axon. (B–D) High power photomicrographs comparing somata among cPAG-projecting RGC (B), alpha cell (C) and melanopsin-expressing RGC (D). (E–G) High power photomicrographs comparing axons among cPAG-projecting RGC (E), alpha cell (F) and melanopsin-expressing RGC (G). The white frames highlight parts of their axons, respetively. Scale bars: 20 µm in A; 10 µm in B (applies to C and D); 5 µm in E (applies to F and G).

Article Snippet: Then retinas were incubated for 3 days at 4°C with a rabbit anti-melanopsin antibody (PA1-780, Fisher Scientific Inc., Barrington, IL; 1∶600).

Techniques: Expressing